Journal: Nature Medicine

Article Title: Immune profiling in a living human recipient of a gene-edited pig kidney

doi: 10.1038/s41591-025-04053-3

Figure Lengend Snippet: a , Integrative clustering of clinical, proteomic and metabolomic data using Fuzzy C-Means clustering across all time points. Normalized multi-omics revealed analytes that increased after xenotransplant despite the administered immunosuppressive regimen. b , Network analysis integrating proteomic and metabolomic data shows several modules enriched for innate immunity pathways. c , Experimental scheme showing that recipient PBMCs obtained at multiple time points were stimulated for 24 h with a regimen of TLR ligands (LPS, Pam3CSK4, R848 and p:IC), following which cytokine concentrations were measured in supernatants using a Luminex device. d – g , Longitudinal concentrations of IL-1β ( d ), IL-6 ( e ), IL-8 ( f ) and GM-CSF ( g ) following TLR ligand stimulation.

Article Snippet: Cells were stimulated with 100 μl of either complete RPMI (controls) or a mixture of bacterial and viral TLR ligands (0.025 μg ml −1 LPS, 10 μg ml −1 Pam3CSK4, 4 μg ml −1 R848, 25 μg ml −1 poly I:C—all from Invivogen).

Techniques: Metabolomic, Biomarker Discovery, Luminex

Journal: Molecular Brain

Article Title: Differential microglial responses to structurally distinct alpha-synuclein polymorphs

doi: 10.1186/s13041-025-01256-0

Figure Lengend Snippet: TLR agonist ability of α-synuclein oligomers. A Schematic illustration of experiment. HEK-Blue hTLR reporter cells were treated with low (100 nM) and high (1 μM) concentrations of α-synuclein oligomers and α-synuclein MO. B – H After 24 h of incubation, the activities of TLR2 ( B ), TLR3 ( C ), TLR4 ( D ), TLR5 ( E ), TLR7 ( F ), TLR8 ( G ), and TLR9 ( H ) were determined by TLR activity reporter assay ( n = 6 per group). Vehicle and TLR agonist were used as negative and positive controls, respectively. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: To determine TLR agonist effect of α-synuclein aggregates, HEK-Blue hTLR reporter cells and HEK-Blue TLR reporter assay were utilized (InvivoGen).

Techniques: Incubation, Activity Assay, Reporter Assay, Comparison

Journal: Molecular Brain

Article Title: Differential microglial responses to structurally distinct alpha-synuclein polymorphs

doi: 10.1186/s13041-025-01256-0

Figure Lengend Snippet: TLR induction ability of α-synuclein fibrillar species. A Experimental scheme. HEK-Blue hTLR reporter cells were treated with low (100 nM) and high (1 μM) concentrations of α-synuclein PFF and α-synuclein sPFF. B–H The activities of TLR2 ( B ), TLR3 ( C ), TLR4 ( D ), TLR5 ( E ), TLR7 ( F ), TLR8 ( G ), and TLR9 ( H ) were determined by TLR activity reporter assay after a 24-h incubation ( n = 6 per group). Vehicle and TLR agonist were used as negative and positive controls, respectively. Error bars represent SEM. One-way ANOVA and Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01

Article Snippet: To determine TLR agonist effect of α-synuclein aggregates, HEK-Blue hTLR reporter cells and HEK-Blue TLR reporter assay were utilized (InvivoGen).

Techniques: Activity Assay, Reporter Assay, Incubation, Comparison